| Product name | Endoplasmin Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human Endoplasmin at AA range: 730-810 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | HSP90B1; GRP94; TRA1; Endoplasmin; 94 kDa glucose-regulated protein; GRP-94; Heat shock protein 90 kDa beta member 1; Tumor rejection antigen 1; gp96 homolog |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 92kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | HSP90B1 encodes a member of a family of adenosine triphosphate (ATP) -metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein (heat shock protein 90 beta family member 1) is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. |
| Gene ID | 7184 |
| Alternative | HSP90B1; GRP94; TRA1; Endoplasmin; 94 kDa glucose-regulated protein; GRP-94; Heat shock protein 90 kDa beta member 1; Tumor rejection antigen 1; gp96 homolog |
| Others | Endoplasmin Polyclonal Antibody detects endogenous levels of Endoplasmin protein. |
| Accession | P14625 |
| Observed Band(KD) | 92 |
Fig.1. Western Blot analysis of various cells using Endoplasmin Polyclonal Antibody diluted at 1:1000.
Fig.2. Western Blot analysis of HeLa cells using Endoplasmin Polyclonal Antibody diluted at 1:1000.
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