| Product name | EKLF/CKLF/UKLF Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human EKLF/CKLF/UKLF at AA range: 280-360 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | KLF1; EKLF; Krueppel-like factor 1; Erythroid krueppel-like transcription factor; EKLF; KLF5; BTEB2; CKLF; IKLF; Krueppel-like factor 5; Basic transcription element-binding protein 2; BTE-binding protein 2; Colon krueppel-like factor; GC-bo |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 38kD(EKLF) 20kD(CKLF/UKLF) |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | KLF1 encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in KLF1 result in the dominant In (Lu) blood phenotype. |
| Gene ID | 10661/688/8609 |
| Alternative | KLF1; EKLF; Krueppel-like factor 1; Erythroid krueppel-like transcription factor; EKLF; KLF5; BTEB2; CKLF; IKLF; Krueppel-like factor 5; Basic transcription element-binding protein 2; BTE-binding protein 2; Colon krueppel-like factor; GC-bo |
| Others | EKLF/CKLF/UKLF Polyclonal Antibody detects endogenous levels of EKLF/CKLF/UKLF protein. |
| Accession | Q13351/Q13887/O75840 |
| Observed Band(KD) | 38 |
Fig.1. Western Blot analysis of various cells using EKLF/CKLF/UKLF Polyclonal Antibody diluted at 1:500.
Fig.2. Western Blot analysis of VEC cells using EKLF/CKLF/UKLF Polyclonal Antibody diluted at 1:500.
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