| Product name | EGF Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from part region of human EGF protein at amino acid sequence of 1000-1060 |
| Host | Rabbit |
| Reactivity | Human,Mouse, |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;IHC 1:500-200;ELISA 1:10000-20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | Pro-epidermal growth factor; EGF; Epidermal growth factor; Urogastrone |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 130kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | EGF encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in EGFare the cause of hypomagnesemia type 4. Dysregulation of EGF has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. |
| Gene ID | 1950 |
| Alternative | Pro-epidermal growth factor; EGF; Epidermal growth factor; Urogastrone |
| Others | The antibody detects endogenous levels of EGF |
| Accession | P01133 |
Fig.1. Western blot analysis of 293T Mouse-kidney lysate, antibody was diluted at 1:2000. HRP, Goat Anti-Rabbit IgG (Cat #: A21020) secondary antibody was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-kidney-cancer, antibody was diluted at 1:200.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-kidney-cancer, antibody was diluted at 1:200.
Fig.4. Immunohistochemical analysis of paraffin-embedded human-stomach-cancer, antibody was diluted at 1:200.
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