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Eg5 (phospho Thr926) Polyclonal Antibody

Eg5 (phospho Thr926) Polyclonal Antibody

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Specification

Product name Eg5 (phospho Thr926) Polyclonal Antibody
Immunogen Synthesized peptide derived from human Eg5 around the phosphorylation site of T926
Host Rabbit
Reactivity Human,Rat,Mouse,
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;ELISA 1:5000;IF 1:50-200
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative KIF11; EG5; KNSL1; TRIP5; Kinesin-like protein KIF11; Kinesin-like protein 1; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 119kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background KIF11 encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.
Gene ID 3832
Alternative KIF11; EG5; KNSL1; TRIP5; Kinesin-like protein KIF11; Kinesin-like protein 1; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5
Others Phospho-Eg5 (T926) Polyclonal Antibody detects endogenous levels of Eg5 protein only when phosphorylated at T926.
Accession P52732
Observed Band(KD) 119

Image & description

Fig.1. Western Blot analysis of COLO205 cells using Phospho-Eg5 (T926) Polyclonal Antibody diluted at 1:2000.

Fig.1. Western Blot analysis of COLO205 cells using Phospho-Eg5 (T926) Polyclonal Antibody diluted at 1:2000.

Fig.2. Western blot analysis of HELA KB SH-SY5Y 293T 3T3 lysis using Phospho-Eg5 (T926) antibody. Antibody was diluted at 1:2000.

Fig.2. Western blot analysis of HELA KB SH-SY5Y 293T 3T3 lysis using Phospho-Eg5 (T926) antibody. Antibody was diluted at 1:2000.

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