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EF-1 α1/2 (Acetyl Lys41) Polyclonal Antibody

EF-1 α1/2 (Acetyl Lys41) Polyclonal Antibody

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Specification

Product name EF-1 α1/2 (Acetyl Lys41) Polyclonal Antibody
Immunogen Synthesized peptide derived from the human EF-1 α1/2 around the acetylation site of K41
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative EEF1A1; EEF1A; EF1A; LENG7; Elongation factor 1-alpha 1; EF-1-alpha-1; Elongation factor Tu; EF-Tu; Eukaryotic elongation factor 1 A-1; eEF1A-1; Leukocyte receptor cluster member 7; EEF1A2; EEF1AL; STN; Elongation factor 1-alpha 2; EF-1-alpha-2;Eukaryotic elongation factor 1 A-2; eEF1A-2; Statin-S1; EEF1A1P5; EEF1AL3; Putative elongation factor 1-alpha-like 3; EF-1-alpha-like 3; Eukaryotic elongation factor 1 A-like 3; eEF1A-like 3; Eukaryotic translation elongation factor 1 alpha-1 pseudogene 5

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 50kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background EEF1A1 encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. eukaryotic translation elongation factor 1 alpha 1 is identified as an autoantigen in 66% of patients with Felty syndrome. EEF1A1 has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes.
Gene ID 1915
Alternative EEF1A1; EEF1A; EF1A; LENG7; Elongation factor 1-alpha 1; EF-1-alpha-1; Elongation factor Tu; EF-Tu; Eukaryotic elongation factor 1 A-1; eEF1A-1; Leukocyte receptor cluster member 7; EEF1A2; EEF1AL; STN; Elongation factor 1-alpha 2; EF-1-alpha-2;Eukaryotic elongation factor 1 A-2; eEF1A-2; Statin-S1; EEF1A1P5; EEF1AL3; Putative elongation factor 1-alpha-like 3; EF-1-alpha-like 3; Eukaryotic elongation factor 1 A-like 3; eEF1A-like 3; Eukaryotic translation elongation factor 1 alpha-1 pseudogene 5
Others Acetyl-EF-1 α1/2 (K41) Polyclonal Antibody detects endogenous levels of EF-1 α1/2 protein only when acetylated at K41.
Accession P68104
Observed Band(KD) 50

Image & description

Fig.1. Western Blot analysis of A549 cells using Acetyl-EF-1 α1/2 (K41) Polyclonal Antibody. Secondary Antibody  was diluted at 1:20000.

Fig.1. Western Blot analysis of A549 cells using Acetyl-EF-1 α1/2 (K41) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.

Fig.2. Western Blot analysis of A549 cells using Acetyl-EF-1 α1/2 (K41) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.

Fig.2. Western Blot analysis of A549 cells using Acetyl-EF-1 α1/2 (K41) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.

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