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E2F-6 Polyclonal Antibody

E2F-6 Polyclonal Antibody

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Specification

Product name E2F-6 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human E2F-6 at AA range: 110-190
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative E2F6; Transcription factor E2F6; E2F-6

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 31kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background E2F6 encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. E2F transcription factor 6 encoded by E2F6 lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants.
Gene ID 1876
Alternative E2F6; Transcription factor E2F6; E2F-6
Others E2F-6 Polyclonal Antibody detects endogenous levels of E2F-6 protein.
Accession O75461
Observed Band(KD) 31

Image & description

Fig.1. Western Blot analysis of various cells using E2F-6 Polyclonal Antibody diluted at 1:500.

Fig.1. Western Blot analysis of various cells using E2F-6 Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of HepG2 cells using E2F-6 Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of HepG2 cells using E2F-6 Polyclonal Antibody diluted at 1:500.

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