| Product name | E2F-3 (Acetyl-Lys168) Polyclonal Antibody |
| Immunogen | Synthetic peptide from human protein at AA range: 130-200 |
| Host | Rabbit |
| Reactivity | Human:K168,Mouse:K160,Rat:K161 |
| Applications | WB,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-10000;ELISA 1:10000 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Alternative | E2F3 KIAA0075 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 50kD |
| Storage buffer | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | E2F3 (E2F transcription factor 3) encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. |
| Gene ID | 1871 |
| Alternative | E2F3 KIAA0075 |
| Others | The antibody detects endogenous E2F-3 (Acetyl-Lys168) protein. |
| Accession | O00716 |
| Observed Band(KD) | 50 |
Fig. Western Blot analysis of 3T3 cells using Antibody diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
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