| Product name | E-Selectin Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from E-Selectin at AA range: INTERNAL |
| Host | Rabbit |
| Reactivity | Human,Rat,Mouse, |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC: 100-300;WB 1:500-2000;ELISA 1:10000-20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | selectin E |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 66kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The protein encoded by SELE (selectin E) is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. |
| Gene ID | 6401 |
| Alternative | selectin E |
| Others | E-Selectin Polyclonal Antibody detects endogenous levels of E-Selectin. |
| Accession | P16581 |
| Observed Band(KD) | 66 |
Fig.1. Western Blot analysis of K562 SH-SY5Y Mouse-brain cells using E-Selectin Polyclonal Antibody diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.
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