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DQB2 Polyclonal Antibody

DQB2 Polyclonal Antibody

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Specification

Product name DQB2 Polyclonal Antibody
Immunogen Synthesized peptide derived from part region of human DQB2 protein
Host Rabbit
Reactivity Human
Applications WB,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;ELISA 1:5000-20000
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative HLA class II histocompatibility antigen, DQ beta 2 chain; HLA class II histocompatibility antigen, DX beta chain; MHC class II antigen DQB2

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 29kD
Storage buffer PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background HLA-DQB2 belongs to the family of HLA class II beta chain paralogs. Class II molecules are heterodimers consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. They play a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: b lymphocytes, dendritic cells, macrophages). Polymorphisms in the alpha and beta chains specify the peptide binding specificity, and typing for these polymorphisms is routinely done for bone marrow transplantation. However this gene, HLA-DQB2, is not routinely typed, as it is not thought to have an effect on transplantation. There is conflicting evidence in the literature and public sequence databases for the protein-coding capacity of HLA-DQB2. Because there is evidence of transcription and an intact ORF, HLA-DQB2 is represented in Entrez Gene and in RefSeq as a protein-coding locus.
Gene ID 3120
Alternative HLA class II histocompatibility antigen, DQ beta 2 chain; HLA class II histocompatibility antigen, DX beta chain; MHC class II antigen DQB2
Others The antibody detects endogenous levels of DQB2 protein
Accession P05538
Observed Band(KD) 29

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