| Product name | DNA Ligase I Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the N-terminal region of human DNA Ligase I at AA range: 80-160 |
| Host | Rabbit |
| Reactivity | Human,Rat,Mouse, |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | LIG1; DNA ligase 1; DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 133kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | LIG1 encodes a member of the ATP-dependent DNA ligase protein family. DNA ligase 1 functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of LIG1 may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. |
| Gene ID | 3978 |
| Alternative | LIG1; DNA ligase 1; DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1 |
| Others | DNA Ligase I Polyclonal Antibody detects endogenous levels of DNA Ligase I protein. |
| Accession | P18858 |
| Observed Band(KD) | 133 |
Fig. Western Blot analysis of various cells using DNA Ligase I Polyclonal Antibody diluted at 1:2000.
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