| Product name | DGK-θ Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human DGK-θ at AA rangle: 660-740 |
| Host | Rabbit |
| Reactivity | Human,Rat,Mouse, |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | DGKQ; DAGK4; Diacylglycerol kinase theta; DAG kinase theta; Diglyceride kinase theta; DGK-theta |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 101kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Diacylglycerol kinase theta encoded by DGKQ contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. |
| Gene ID | 1609 |
| Alternative | DGKQ; DAGK4; Diacylglycerol kinase theta; DAG kinase theta; Diglyceride kinase theta; DGK-theta |
| Others | DGK-θ Polyclonal Antibody detects endogenous levels of DGK-θ protein. |
| Accession | P52824 |
| Observed Band(KD) | 101 |
Fig. Western Blot analysis of HT-29 cells using DGK-θ Polyclonal Antibody.
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