| Product name | DDX52 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human DDX52 at AA range: 530-610 |
| Host | Rabbit |
| Reactivity | Human,Rat,Mouse, |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | DDX52; ROK1; HUSSY-19; Probable ATP-dependent RNA helicase DDX52; ATP-dependent RNA helicase ROK1-like; DEAD box protein 52 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 67kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DEAD box protein 52 (DDX52), also known as ATP-dependent RNA helicase ROK1-like or HUSSY-19, is a 599 amino acid protein belonging to the DEAD box helicase family. Localized to the nucleus, DDX52 is phosphorylated by ATM or ATR upon DNA damage. DDX52 contains one helicase ATP-binding domain and one helicase C-terminal domain. |
| Gene ID | 11056 |
| Alternative | DDX52; ROK1; HUSSY-19; Probable ATP-dependent RNA helicase DDX52; ATP-dependent RNA helicase ROK1-like; DEAD box protein 52 |
| Others | DDX52 Polyclonal Antibody detects endogenous levels of DDX52 protein. |
| Accession | Q9Y2R4 |
| Observed Band(KD) | 67 |
Fig. Western Blot analysis of various cells using DDX52 Polyclonal Antibody diluted at 1:500.
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