| Product name | DAP-5 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the N-terminal region of human DAP-5 at AA range: 10-90 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | EIF4G2; DAP5; OK/SW-cl.75; Eukaryotic translation initiation factor 4 gamma 2; eIF-4-gamma 2; eIF-4G 2; eIF4G 2; Death-associated protein 5; DAP-5; p97 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 90kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. Eukaryotic translation initiation factor 4 gamma 2 encoded by EIF4G2 shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, EIF4G2 product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of EIF4G2 have been described. |
| Gene ID | 1982 |
| Alternative | EIF4G2; DAP5; OK/SW-cl.75; Eukaryotic translation initiation factor 4 gamma 2; eIF-4-gamma 2; eIF-4G 2; eIF4G 2; Death-associated protein 5; DAP-5; p97 |
| Others | DAP-5 Polyclonal Antibody detects endogenous levels of DAP-5 protein. |
| Accession | P78344 |
| Observed Band(KD) | 90 |
Fig.1. Western Blot analysis of various cells using DAP-5 Polyclonal Antibody.
Fig.2. Western Blot analysis of A549 cells using DAP-5 Polyclonal Antibody.
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