| Product name | DAAM1 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from part region of human DAAM1 protein at amino acid sequence of 400-500 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;ELISA 1:10000-20000 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | Disheveled-associated activator of morphogenesis 1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 123kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Cell motility, adhesion, cytokinesis, and other functions of the cell cortex are mediated by reorganization of the actin cytoskeleton and several formin homology (FH) proteins have been associated with these processes. The protein encoded by DAAM1 contains two FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. A key regulator of cytoskeletal architecture, the small GTPase Rho, is activated during development by Wnt/Fz signaling to control cell polarity and movement. The protein encoded by DAAM1 is thought to function as a scaffolding protein for the Wnt-induced assembly of a disheveled (Dvl)-Rho complex. This protein also promotes the nucleation and elongation of new actin filaments and regulates cell growth through the stabilization of microtubules. Alternative splicing results in multiple transcript variants encoding distinct proteins. |
| Gene ID | 23002 |
| Alternative | Disheveled-associated activator of morphogenesis 1 |
| Others | The antibody detects endogenous levels of DAAM1 |
| Accession | Q9Y4D1 |
| Observed Band(KD) | 123 |
Fig.1. Western blot analysis of SW480 MCF7 lysate, antibody was diluted at 1:500. HRP, Goat Anti-Rabbit IgG (Cat #: A21020) secondary antibody was diluted at 1:20000.
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