| Product name | Cytokeratin 20 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human Cytokeratin 20 at AA range: 350-430 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | KRT20; Keratin; type I cytoskeletal 20; Cytokeratin-20; CK-20; Keratin-20; K20; Protein IT |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 49kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Keratin 20 encoded by KRT20 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. |
| Gene ID | 54474 |
| Alternative | KRT20; Keratin; type I cytoskeletal 20; Cytokeratin-20; CK-20; Keratin-20; K20; Protein IT |
| Others | Cytokeratin 20 Polyclonal Antibody detects endogenous levels of Cytokeratin 20 protein. |
| Accession | P35900 |
| Observed Band(KD) | 49 |
Fig. Western Blot analysis of various cells using Cytokeratin 20 Polyclonal Antibody diluted at 1:500.
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