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Cytokeratin 14 Polyclonal Antibody

Cytokeratin 14 Polyclonal Antibody

Views(21) Publications(0) Catalog no(ABP52904)
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Specification

Product name Cytokeratin 14 Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human Cytokeratin 14
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:20000;IF 1:50-200
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative KRT14; Keratin, type I cytoskeletal 14; Cytokeratin-14; CK-14; Keratin-14; K14

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 53kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background KRT14 encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.
Gene ID 3861
Alternative KRT14; Keratin, type I cytoskeletal 14; Cytokeratin-14; CK-14; Keratin-14; K14
Others Cytokeratin 14 Polyclonal Antibody detects endogenous levels of Cytokeratin 14 protein.
Accession P02533
Observed Band(KD) 53

Image & description

Fig.1. Western Blot analysis of MCF7 cells using Cytokeratin 14 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of MCF7 cells using Cytokeratin 14 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded Mouse-brain, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded Mouse-brain, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded Mouse-brain, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded Mouse-brain, antibody was diluted at 1:100.

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