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Cytokeratin 10 Polyclonal Antibody

Cytokeratin 10 Polyclonal Antibody

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Specification

Product name Cytokeratin 10 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human Cytokeratin 10 at AA range: 110-190
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative KRT10; KPP; Keratin; type I cytoskeletal 10; Cytokeratin-10; CK-10; Keratin-10; K10

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 59kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background KRT10 encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in KRT10 are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.
Gene ID 3858
Alternative KRT10; KPP; Keratin; type I cytoskeletal 10; Cytokeratin-10; CK-10; Keratin-10; K10
Others Cytokeratin 10 Polyclonal Antibody detects endogenous levels of Cytokeratin 10 protein.
Accession P13645
Observed Band(KD) 59

Image & description

Fig.1. Western Blot analysis of various cells using Cytokeratin 10 Polyclonal Antibody.

Fig.1. Western Blot analysis of various cells using Cytokeratin 10 Polyclonal Antibody.

Fig.2. Western Blot analysis of HeLa cells using Cytokeratin 10 Polyclonal Antibody.

Fig.2. Western Blot analysis of HeLa cells using Cytokeratin 10 Polyclonal Antibody.

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