| Product name | Cytochrome b5 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human Cytochrome b5 at AA range: 30-110 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:5000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | CYB5A; CYB5; Cytochrome b5; Microsomal cytochrome b5 type A; MCB5 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 15kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Cytochrome b5 type A encoded by CYB5A is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in CYB5A are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for CYB5A. |
| Gene ID | 1528 |
| Alternative | CYB5A; CYB5; Cytochrome b5; Microsomal cytochrome b5 type A; MCB5 |
| Others | Cytochrome b5 Polyclonal Antibody detects endogenous levels of Cytochrome b5 protein. |
| Accession | P00167 |
| Observed Band(KD) | 15 |
Fig. Western Blot analysis of various cells using Cytochrome b5 Polyclonal Antibody.
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