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Cytochrome b Polyclonal Antibody

Cytochrome b Polyclonal Antibody

Views(11) Publications(1) Catalog no(ABP58323)
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Specification

Product name Cytochrome b Polyclonal Antibody
Immunogen Synthesized peptide derived from part region of human Cytochrome b protein at amino acid sequence of 331-380
Host Rabbit
Reactivity Human,Mouse
Applications WB,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;ELISA 1:10000-20000
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Cytochrome b; Complex III subunit 3; Complex III subunit III; Cytochrome b-c1 complex subunit 3; Ubiquinol-cytochrome-c reductase complex cytochrome b subunit

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 48kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MT-CYB (Mitochondrially Encoded Cytochrome B) is a Protein Coding gene. Diseases associated with MT-CYB include Infantile Histiocytoid Cardiomyopathy and Leber Optic Atrophy. Among its related pathways are Metabolism and Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins.
Gene ID 4519
Alternative Cytochrome b; Complex III subunit 3; Complex III subunit III; Cytochrome b-c1 complex subunit 3; Ubiquinol-cytochrome-c reductase complex cytochrome b subunit
Others The antibody detects endogenous levels of Cytochrome b
Accession P00156
Observed Band(KD) 48

Image & description

Fig.1. Western Blot analysis of 1, Mouse-lung 2, Mouse-brain 3,Mouse-spleen 4,Mouse-kidney 5,Mouse-heart cells using primary antibody diluted at 1:500 (4°C overnight). Goat Anti-rabbit IgG Dylight 800 (Cat #: A23920) secondary antibody was diluted at 1:5000 at 25°C for 1 hour.

Fig.1. Western Blot analysis of 1, Mouse-lung 2, Mouse-brain 3,Mouse-spleen 4,Mouse-kidney 5,Mouse-heart cells using primary antibody diluted at 1:500 (4°C overnight). Goat Anti-rabbit IgG Dylight 800 (Cat #: A23920) secondary antibody was diluted at 1:5000 at 25°C for 1 hour.

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