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CYP2A6 Polyclonal Antibody

CYP2A6 Polyclonal Antibody

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Specification

Product name CYP2A6 Polyclonal Antibody
Immunogen Synthesized peptide derived from the N-terminal region of human CYP2A6 at AA rangle: 10-90
Host Rabbit
Reactivity Human
Applications WB,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative CYP2A6; CYP2A3; Cytochrome P450 2A6; 1; 4-cineole 2-exo-monooxygenase; CYPIIA6; Coumarin 7-hydroxylase; Cytochrome P450 IIA3; Cytochrome P450(I)

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 56kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background This gene, CYP2A6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to hydroxylate coumarin, and also metabolizes nicotine, aflatoxin B1, nitrosamines, and some pharmaceuticals. Individuals with certain allelic variants are said to have a poor metabolizer phenotype, meaning they do not efficiently metabolize coumarin or nicotine. This gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. The gene was formerly referred to as CYP2A3; however, it has been renamed CYP2A6.
Gene ID 1548
Alternative CYP2A6; CYP2A3; Cytochrome P450 2A6; 1; 4-cineole 2-exo-monooxygenase; CYPIIA6; Coumarin 7-hydroxylase; Cytochrome P450 IIA3; Cytochrome P450(I)
Others CYP2A6 Polyclonal Antibody detects endogenous levels of CYP2A6 protein.
Accession P11509
Observed Band(KD) 56

Image & description

Fig. Western Blot analysis of PC-12 cells using CYP2A6 Polyclonal Antibody diluted at 1:1000.

Fig. Western Blot analysis of PC-12 cells using CYP2A6 Polyclonal Antibody diluted at 1:1000.

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