| Product name | Cyclophilin F Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human Cyclophilin F at AA range: 60-140 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | PPIF; CYP3; Peptidyl-prolyl cis-trans isomerase F; mitochondrial; PPIase F; Cyclophilin F; Rotamase F |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 22kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Peptidylprolyl isomerase F encoded by PPIF is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. |
| Gene ID | 10105 |
| Alternative | PPIF; CYP3; Peptidyl-prolyl cis-trans isomerase F; mitochondrial; PPIase F; Cyclophilin F; Rotamase F |
| Others | Cyclophilin F Polyclonal Antibody detects endogenous levels of Cyclophilin F protein. |
| Accession | P30405 |
| Observed Band(KD) | 22 |
Fig.1. Western Blot analysis of various cells using Cyclophilin F Polyclonal Antibody diluted at 1:2000.
Fig.2. Western Blot analysis of HuvEc cells using Cyclophilin F Polyclonal Antibody diluted at 1:2000.
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