| Product name | Cyclin E1 (phospho Thr395) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human Cyclin E1 around the phosphorylation site of T395 |
| Host | Rabbit |
| Reactivity | Human,Rat,Mouse, |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | CCNE1; CCNE; G1/S-specific cyclin-E1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 48kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Cyclin E1 encoded by CCNE1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E1 accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of CCNE1 has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. Cyclin E1 was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. |
| Gene ID | 898 |
| Alternative | CCNE1; CCNE; G1/S-specific cyclin-E1 |
| Others | Phospho-Cyclin E1 (T395) Polyclonal Antibody detects endogenous levels of Cyclin E1 protein only when phosphorylated at T395. |
| Accession | P24864 |
| Observed Band(KD) | 48 |
Fig.1. Western Blot analysis of various cells using Phospho-Cyclin E1 (T395) Polyclonal Antibody diluted at 1:2000.
Fig.2. Western Blot analysis of HeLa cells using Phospho-Cyclin E1 (T395) Polyclonal Antibody diluted at 1:2000.
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