| Product name | CYB5R3 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human CYB5R3 at AA range: 110-190 |
| Host | Rabbit |
| Reactivity | Human,Rat,Mouse, |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | CYB5R3; DIA1; NADH-cytochrome b5 reductase 3; B5R; Cytochrome b5 reductase; Diaphorase-1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 34kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | CYB5R3 encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in CYB5R3 cause methemoglobinemias. |
| Gene ID | 1727 |
| Alternative | CYB5R3; DIA1; NADH-cytochrome b5 reductase 3; B5R; Cytochrome b5 reductase; Diaphorase-1 |
| Others | CYB5R3 Polyclonal Antibody detects endogenous levels of CYB5R3 protein. |
| Accession | P00387 |
| Observed Band(KD) | 34 |
Fig. Western Blot analysis of various cells using CYB5R3 Polyclonal Antibody.
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