| Product name | Csk Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human Csk around the non-phosphorylation site of S364 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | CSK; Tyrosine-protein kinase CSK; C-Src kinase; Protein-tyrosine kinase CYL |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 50kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | All members of the Src gene family of tyrosine kinases are characterized by a carboxy terminal domain tyrosine which is highly phosphorylated in the inactive form of the enzyme and phosphorylated to a much lesser extent when the enzyme is active. In the case of Src p60, Y527 is this tyrosine; however, a mutant form of c-Src in which Y527 is replaced by phenylalanine is transforming and displays 5- to 10-fold elevated kinase activity compared to its normal counterpart. Csk has been identified as a Src-related tyrosine kinase having both SH2 and SH3 domains and a catalytic domain but lacking sequences amino terminal to the SH3 domain as well as carboxy terminal regulatory sequences. Csk phosphorylates Src on Y527 and also downregulates Lyn, Fyn and Lck by tyrosine phosphorylation of carboxy terminal regulatory sites. |
| Gene ID | 1445 |
| Alternative | CSK; Tyrosine-protein kinase CSK; C-Src kinase; Protein-tyrosine kinase CYL |
| Others | Csk Polyclonal Antibody detects endogenous levels of Csk protein. |
| Accession | P41240 |
| Observed Band(KD) | 50 |
Fig.1. Western Blot analysis of various cells using Csk Polyclonal Antibody diluted at 1:1000.
Fig.2. Western Blot analysis of 22RV1 cells using Csk Polyclonal Antibody diluted at 1:1000.
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