| Product name | CREB-1 (phospho Ser133) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human CREB-1 around the phosphorylation site of S133 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | ELISA, IF, IHC-P, IP, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:50-1:200), IHC-P (1:100-1:300), IP (2-5 ug/mg lysate), ELISA (1:10000). Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Alternative | CREB1; Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 37 KD |
| Storage buffer | PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | CREB1 encodes a transcription factor (cAMP responsive element binding protein 1) that is a member of the leucine zipper family of DNA binding proteins. cAMP responsive element binding protein 1 binds as a homodimer to the cAMP-responsive element, an octameric palindrome. cAMP responsive element binding protein 1 is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of CREB1 results in several transcript variants encoding different isoforms. |
| Gene ID | 1385 |
| Alternative | CREB1; Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1 |
| Others | Phospho-CREB-1 (S133) Polyclonal Antibody detects endogenous levels of CREB-1 protein only when phosphorylated at S133. |
| Accession | P16220 |
Fig.1. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, CREB-1 (phospho Ser133) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.2. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, CREB-1 (phospho Ser133) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.3. Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1, CREB-1 (phospho Ser133) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.4. Western Blot analysis of MCF-7 (1), A549 (2), K562 (3), HEK293 (4), diluted at 1:1000.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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