| Product name | Cortactin (phospho Tyr466) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human Cortactin around the phosphorylation site of Y466 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat,Monkey |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | CTTN; EMS1; Src substrate cortactin; Amplaxin; Oncogene EMS1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 80kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Cortactin, encoded by CTTN gene (formerly EMS1), was originally identified as a substrate of the Src kinase. It is a cytoskeletal protein that interacts with F-actin and Arp2/3 complex and plays an important role in regulation of actin assembly and cytoskeleton dynamics. Overexpression of cortactin increases cell migration, and is thought to contribute to the invasive phennotype of breast cancer and several other tumors. Three isoforms of cortactin exist due to the alternative splicing. |
| Gene ID | 13043 |
| Alternative | CTTN; EMS1; Src substrate cortactin; Amplaxin; Oncogene EMS1 |
| Others | Phospho-Cortactin (Y466) Polyclonal Antibody detects endogenous levels of Cortactin protein only when phosphorylated at Y466. |
| Accession | Q14247 |
| Observed Band(KD) | 80 |
Fig.1. Western Blot analysis of various cells using Phospho-Cortactin (Y466) Polyclonal Antibody diluted at 1:500.
Fig.2. Western Blot analysis of 293 cells using Phospho-Cortactin (Y466) Polyclonal Antibody diluted at 1:500.
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