| Product name | Cortactin (phospho Tyr421) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human Cortactin around the phosphorylation site of Y421 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | CTTN; EMS1; Src substrate cortactin; Amplaxin; Oncogene EMS1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 80kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | CTTN is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The cortactin is localized in the cytoplasm and in areas of the cell-substratum contacts. CTTN has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. |
| Gene ID | 2017 |
| Alternative | CTTN; EMS1; Src substrate cortactin; Amplaxin; Oncogene EMS1 |
| Others | Phospho-Cortactin (Y421) Polyclonal Antibody detects endogenous levels of Cortactin protein only when phosphorylated at Y421. |
| Accession | Q14247 |
| Observed Band(KD) | 80 |
Fig. Western Blot analysis of LOVO+H2O2 cells using Phospho-Cortactin (Y421) Polyclonal Antibody.
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