| Product name | Collagen I Mouse Monoclonal Antibody (4H10) |
| Immunogen | Synthetic Peptide of Collagen I |
| Host | Mouse |
| Reactivity | Human,Mouse,Rat |
| Applications | IF,IHC |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200;IHC 1:50-300 |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | Collagen alpha-1(I) chain; Alpha-1 type I collagen |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 139kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | COL1A1 (collagen type I alpha 1 chain) encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for COL1A1. |
| Gene ID | 1277/1278 |
| Alternative | Collagen alpha-1(I) chain; Alpha-1 type I collagen |
| Others | The antibody detects endogenous Collagen I protein. |
| Accession | P02452 |
| Observed Band(KD) | 139 |
Fig.1. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue. 1, Collagen I Mouse Monoclonal Antibody (4H10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.2. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, Collagen I Mouse Monoclonal Antibody (4H10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Author：M Lecomte, D Tomassi, R Rizzoli, M Tenon, T Berton Publication name：Nutrient IF：5.9
Author：Zhang Y, Li T Publication name：J Transl Med IF：4.124
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