| Product name | COL17A1 Polyclonal Antibody |
| Immunogen | Synthetic peptide from human protein at AA range: 481-530 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;IF 1:100-300;ELISA 1:5000-20000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | COL17A1; BP180; BPAG2 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 150kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | COL17A1 (collagen type XVII alpha 1 chain) encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in COL17A1 are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. |
| Gene ID | 1308 |
| Alternative | COL17A1; BP180; BPAG2 |
| Others | The antibody detects endogenous COL17A1 protein. |
| Accession | Q9UMD9 |
| Observed Band(KD) | 150 |
Fig.1. Western Blot analysis of 3T3, hepg2 cells using Antibody diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:200.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200.
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