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Cleaved-Plasma Kallikrein HC (R390) Polyclonal Antibody

Cleaved-Plasma Kallikrein HC (R390) Polyclonal Antibody

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Specification

Product name Cleaved-Plasma Kallikrein HC (R390) Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human Plasma Kallikrein HC at AA range: 310-390
Host Rabbit
Reactivity Human,Rat,Mouse,
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative KLKB1; KLK3; Plasma kallikrein; Fletcher factor; Kininogenin; Plasma prekallikrein

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 45kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background KLKB1 encodes a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. The encoded preproprotein present in plasma as a non-covalent complex with high molecular weight kininogen undergoes proteolytic processing mediated by activated coagulation factor XII to generate a disulfide-linked, heterodimeric serine protease comprised of heavy and light chains. Certain mutations in KLKB1 cause prekallikrein deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms.
Gene ID 3818
Alternative KLKB1; KLK3; Plasma kallikrein; Fletcher factor; Kininogenin; Plasma prekallikrein
Others Cleaved-Plasma Kallikrein HC (R390) Polyclonal Antibody detects endogenous levels of fragment of activated Plasma Kallikrein HC protein resulting from cleavage adjacent to R390.
Accession P03952
Observed Band(KD) 45

Image & description

Fig.1. Western Blot analysis of various cells using Cleaved-Plasma Kallikrein HC (R390) Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using Cleaved-Plasma Kallikrein HC (R390) Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of HeLa cells using Cleaved-Plasma Kallikrein HC (R390) Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of HeLa cells using Cleaved-Plasma Kallikrein HC (R390) Polyclonal Antibody diluted at 1:1000.

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