| Product name | Cleaved-Lamin A (D230) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human Lamin A at AA range: 150-230 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | LMNA; LMN1; Prelamin-A/C |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 28kD,75kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in LMNA lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. |
| Gene ID | 4000 |
| Alternative | LMNA; LMN1; Prelamin-A/C |
| Others | Cleaved-Lamin A (D230) Polyclonal Antibody detects endogenous levels of fragment of activated Lamin A protein resulting from cleavage adjacent to D230. |
| Accession | P02545 |
| Observed Band(KD) | 28+75 |
Fig. Western Blot analysis of various cells using Cleaved-Lamin A (D230) Polyclonal Antibody.
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