| Product name | CheKine™ Mirco Sucrose Phosphate Synthase (SPS) Activity Assay Kit |
| SampleType | Plant tissues |
| Alternative | SPS |
| Kit components | •Extraction Buffer •Reagent I •Reagent Ⅱ •Reagent Ⅲ •Reagent Ⅳ •Standard |
| Features & Benefits | The verified samples are complete in type and easy to operate． |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
| Storage instructions | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Sucrose is not only an important photosynthetic product but also the primary substance transported within plants and serves as a form of carbohydrate storage. Sucrose phosphate synthase (SPS, EC 2.4.1.14) uses fructose-6-phosphate as a receptor, and the formed sucrose-6-phosphate is converted into sucrose by sucrose phosphate phosphatase. The SPS-sucrose phosphate phosphatase system is generally considered the main pathway for sucrose synthesis. CheKine™ Mirco Sucrose Phosphate Synthase (SPS) Activity Assay Kit provides a simple, convenient, and rapid method for detecting SPS activity, suitable for plant tissue samples. The principle involves SPS catalyzing the formation of sucrose-6-phosphate from fructose-6-phosphate. Sucrose-6-phosphate reacts with resorcinol to produce a color change, exhibiting a characteristic absorption peak at 480 nm, with the intensity of the color being proportional to the level of enzyme activity. |
| Alternative | SPS |
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