| Product name | CheKine™ MicroTotal glutathione （T-GSH）/ Oxidized glutathione（GSSG） Assay Kit |
| SampleType | Serum, Plasma, Animal/Plant Tissues, Whole blood, Blood cells, Cells, Bacteria |
| Alternative | T-GSH），GSSG |
| Kit components | •Extraction Buffer •Inhibitor •Assay Buffer •GR •GR Cofactor •Chromogen •GSH Standard •GSSG Standard |
| Features & Benefits | The verified samples are complete in type and easy to operate． |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
| Storage instructions | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The total Glutathione has two forms: reduced form (GSH) and oxidized form (GSSG). GSSG also known as Dithioglutathione, is formed by the oxidation of two molecules of glutathione. GSSG will be reduced to GSH by glutathione reductase (GR), therefore most of glutathione exist in the reduced form. Determination of intracellular GSH and GSSG content and GSH/GSSG ratio can well reflect the redox state of cells, and is also one of the main indicators of glutathione redox cycle. CheKine™ Micro Total glutathione （T-GSH）/Oxidized glutathione（GSSG） Assay Kit provides a simple, convenient and rapid method for the detection of T-GSH and GSSG content, which is suitable for serum, plasma, animal and plant tissues, whole blood, blood cells, cells, bacteria, etc. sample. The principle is that GSSG was reduced to GSH. The reduced Glutathione can react with 5,5-dithiobis (2-nitrobenzoic acid) (DTNB) to generate 2-nitro-5-mercaptobenzoic acid, which has a maximum absorption at wavelength of 412 nm, thereby determining the content of total GSH by absorbance changes. If the original reduced glutathione in the sample was inhibited by 2-vinylpyridine, and then use GR to reduce GSSG to GSH, thereby determining the content of oxidized glutathione. The reduced glutathione can be calculated by substract GSSG from total GSH. |
| Alternative | T-GSH），GSSG |
Fig.CheKine™ MicroTotal glutathione （T-GSH）/ Oxidized glutathione（GSSG） Assay Kit
You must be logged in to post a review.
Reviews
There are no reviews yet.