Product name | CheKine™ Micro Soil Neutral Invertase (S-NI) Activity Assay Kit |
SampleType | Soil |
Alternative | Soil Neutral Invertase |
Kit components | • ReagentⅠ • ReagentⅡ • ReagentⅢ • Standard |
Features & Benefits | The verified samples are complete in type and easy to operate. |
Calibration range | 0.03215-1 mg/mL |
Limit of detection | 0.03215 mg/mL |
Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
Storage instructions | Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
Shipping | Gel pack with blue ice. |
Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background | Sucrose invertase (Invertase, Ivr) catalyzes the irreversible hydrolysis of sucrose into fructose and glucose, and is one of the key enzymes involved in sucrose metabolism in higher plants. Based on optimal pH, Ivr can be classified into two types: acid invertase (AI) and neutral invertase (NI). CheKine™ Micro Soil Neutral Invertase S-NI) Activity Assay Kit provides a simple, convenient, and rapid method for determining neutral invertase activity in soil samples. The principle of the assay is based on the ability of S-NI to catalyze the hydrolysis of sucrose, producing reducing sugars that subsequently react with 3,5-dinitrosalicylic acid (DNS) to form a red-brown amino compound. This compound exhibits a characteristic absorbance at 540 nm, and the absorbance value is proportional to the amount of reducing sugar produced within a certain range. The activity of S-NI is calculated based on the rate of increase in absorbance at 540 nm. |
Alternative | Soil Neutral Invertase |
Fig.CheKine™ Micro Soil Neutral Invertase (S-NI) Activity Assay Kit
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