| Product name | CheKine™ Micro Soil Acidic Xylanase (S-ACX) Protease Activity Assay Kit |
| SampleType | Soil |
| Alternative | Soil Acidic Xylanase (S-ACX) |
| Kit components | • Reagent I •Reagent Ⅱ •Reagent Ⅲ •Reagent Ⅳ •Reagent V •Reagent VI •Standard |
| Features & Benefits | The verified samples are complete in type and easy to operate． |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
| Storage instructions | Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Xylanase (EC 3.2.1.8) is mainly produced by microorganisms and can catalyze the hydrolysis of xylan. It is also referred to as pentosanase or hemicellulase, capable of breaking down cell walls in raw materials used in brewing or feed industries, as well as β-glucans. This reduces the viscosity of materials during brewing, promotes the release of effective substances, and decreases non-starch polysaccharides in feed, thereby enhancing the absorption and utilization of nutrients. Due to these properties, xylanase is widely applied in brewing and feed industries. Acidic xylanase (ACX) is generally isolated from acid-tolerant fungi, bacteria, and some molds. CheKine™ Micro Soil Acidic Xylanase (S-ACX) Protease Activity Assay Kit provides a simple, convenient, and rapid method for detecting neutral xylanase activity in soil samples. Its principle is based on ACX degrading xylan into reducing oligosaccharides and monosaccharides under acidic conditions. These products further react with 3,5-dinitrosalicylic acid (DNS) in a boiling water bath to produce a colorimetric reaction with a characteristic absorption peak at 540 nm. The intensity of the reaction solution's color is proportional to the amount of reducing sugars produced during enzymatic hydrolysis. By measuring the increase rate of absorbance at 540 nm, the activity of ACX can be calculated. |
| Alternative | Soil Acidic Xylanase (S-ACX) |
Fig.CheKine™ Micro Soil Acidic Xylanase (S-ACX) Protease Activity Assay Kit
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