| Product name | CheKine™ Micro Pyrrolidine-5-Carboxylate Synthase (P5CS) Activity Assay Kit |
| SampleType | Plant tissue |
| Alternative | Pyrrolidine-5-Carboxylate Synthase (P5CS) |
| Kit components | • Extraction Buffer • Reagent I •Reagent Ⅱ •Standard |
| Features & Benefits | The verified samples are complete in type and easy to operate． |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
| Storage instructions | Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Proline is an important osmotic adjustment substance in plants to adapt to adversity stress. Proline metabolism in higher plants is divided into two synthetic pathways: glutamic acid (Glu) and ornithine (Orn) due to different initial substrates. Pyrroline -5- carboxylate synthase (P5CS is a key enzyme in proline synthesis from glutamic acid, which plays a key role in plants' adaptation to adversity stress. CheKine™ Micro Pyrrolidine-5-Carboxylate Synthase (P5CS) Activity Assay Kit can be used to detect biological samples such as plant tissue. In the kit, P5CS catalyzes the decomposition of ATP to produce ADP and inorganic phosphorus in the process of glutamic acid producing P5C. The activity of P5CS can be determined by measuring the amount of inorganic phosphorus produced per unit time by ammonium molybdate colorimetry. |
| Alternative | Pyrrolidine-5-Carboxylate Synthase (P5CS) |
Fig.CheKine™ Micro Pyrrolidine-5-Carboxylate Synthase (P5CS) Activity Assay Kit
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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