| Product name | CheKine™ Micro Lysine (LYS) Assay Kit |
| Applications notes | CheKine™ Micro Lysine (LYS) Assay Kit provides a convenient tool for detection of lysine. The principle is that the lysine in protein has a free ε-NH2, which can react with Ninhydrin hydrate to produce blue-purple substances, which has a maximum absorption peak at 570 nm. The lysine content of the sample can be calculated by measuring the absorbance at 570 nm. |
| Kit components | • Extraction Buffer • Assay Buffer • Substrate • Substrate Cofactor • Standard |
| Features & Benefits | • Determination of Lysine (LYS) conten in a variety of biological samples such as serum, plasma, animal and plant tissues, cells, cell supernatant, bacteria. • A broad range linearity: 0.125-8 μmol/mL • Detailed sample preparation and result calculation methods are provided. • Standard and standard curve line are provided. |
| Calibration range | 0.125-8 μmol/mL |
| Limit of detection | 0.03125 μmol/mL |
| Storage instructions | Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Lysine is one of the essential amino acids of human body, which can promote human development, enhance immune function and improve the function of central nervous system. Lysine is a basic essential amino acid. Because the lysine content in cereal food is very low, and it is easy to be destroyed and lacking during processing, it is called the first restricted amino acid. |
Fig.1 Standard Curve of Lysine in 96-well plate assay-data provided for demonstration purposes only.
Fig.2 Lysine content in Mouse liver and Arabidopsis respectively.
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