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CheKine™ Micro Cysteinyl Sulfoxide Lyase (CSL) Activity Assay Kit

CheKine™ Micro Cysteinyl Sulfoxide Lyase (CSL) Activity Assay Kit

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Specification

Product name CheKine™ Micro Cysteinyl Sulfoxide Lyase (CSL) Activity Assay Kit
SampleType Plant Tissue, Liquid samples
Alternative Cysteinyl Sulfoxide Lyase (CSL)

Product Properties

Kit components • Extraction Buffer
• Reagent I
•Reagent Ⅱ
•Reagent Ⅲ
•Reagent IV
•Reagent V
•Standard
Features & Benefits The verified samples are complete in type and easy to operate.
Usage notes • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples.
Storage instructions Storage at -20℃ and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Cysteinylsulfoxide lyase (CSL), referred to as alliinase, also known as alliinase. Cysteinyl sulfoxide lyase is found in almost all kinds of allium plants, such as garlic, onion, leek, etc. Alliinase is found in the vacuole, and its natural substrate alliinine is present in the cytoplasm; alliinase comes into contact with alliin and catalyzed the production of alliin and cis and trans ahocene and produces by-products such as pyruvate and ammonia, and is also the main source of spicy odors in plants such as garlic. CheKine™ Micro Cysteinyl Sulfoxide Lyase (CSL) Activity Assay Kit can be used to detect biological samples such as plant tissue or liquid samples. In the kit, CSL catalyzed the S-methyl-L-cysteine sulfoxide reaction to produce pyruvate, reacted with 2,4-dinitrobenzene hydrazine, which turned brown-red under alkaline conditions and had characteristic absorption peaks at 510nm.
Alternative Cysteinyl Sulfoxide Lyase (CSL)

Image & description

Fig.CheKine™ Micro Cysteinyl Sulfoxide Lyase (CSL) Activity Assay Kit

Fig.CheKine™ Micro Cysteinyl Sulfoxide Lyase (CSL) Activity Assay Kit

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