| Product name | CheKine™ Micro ATP Citrate Lyase (ACL) Activity Assay Kit |
| SampleType | Animal and plant tissues, bacteria and cells, serum or plasma |
| Alternative | ATP Citrate Lyase (ACL) |
| Kit components | •Extraction Buffer •Reagent I •Reagent Ⅱ •Reagent Ⅲ |
| Features & Benefits | The verified samples are complete in type and easy to operate． |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
| Storage instructions | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | ATP citrate lyase is a key cytosolic enzyme that catalyzes the production of acetyl-CoA from citric acid. Produced acetyl-CoA is the main raw material for the synthesis of fatty substances such as fatty acids and cholesterol, and can participate in the modification of related important proteins. It is a pivotal substance for energy substance metabolism in the body. CheKine™ Micro ATP Citrate Lyase (ACL) Activity Assay Kit can be used to detect biological samples such as animal and plant tissue, cells or bacteria, serum or plasma. In this kit, in the presence of ATP and coenzyme A, ACL can catalyze the cleavage of citric acid into acetyl coenzyme A, oxaloacetate, ADP, and phosphate. Malate dehydrogenase further catalyzes oxaloacetate and NADH to produce malate and NAD+, leading light absorption decreases at 340 nm. |
| Alternative | ATP Citrate Lyase (ACL) |
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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