| Product name | Cdk6 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from part region of human Cdk6 protein at amino acid sequence of 280-325 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;IHC 1:500-200;ELISA 1:10000-20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | Cyclin-dependent kinase 6; Cell division protein kinase 6; Serine/threonine-protein kinase PLSTIRE |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 34kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Cdk6 protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of Cdk6 protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly. |
| Gene ID | 1021 |
| Alternative | Cyclin-dependent kinase 6; Cell division protein kinase 6; Serine/threonine-protein kinase PLSTIRE |
| Others | The antibody detects endogenous levels of Cdk6 |
| Accession | Q00534 |
| Observed Band(KD) | 34 |
Fig.1. Western blot analysis of 293T Hela lysate, antibody was diluted at 1:2000. HRP, Goat Anti-Rabbit IgG (Cat #: A21020) secondary antibody was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200.
Fig.4. Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:200.
Fig.5. Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:200.
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