| Product name | Cdc25C (phospho Ser216) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human Cdc25C around the phosphorylation site of S216 |
| Host | Rabbit |
| Reactivity | Human,Rat,Mouse, |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | CDC25C; M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 53kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | CDC25C encodes a conserved protein (cell division cycle 25C) that plays a key role in the regulation of cell division. Cell division cycle 25C directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of CDC25C have been described. |
| Gene ID | 995 |
| Alternative | CDC25C; M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C |
| Others | Phospho-Cdc25C (S216) Polyclonal Antibody detects endogenous levels of Cdc25C protein only when phosphorylated at S216. |
| Accession | P30307 |
| Observed Band(KD) | 53 |
Fig.1. Western Blot analysis of various cells using Phospho-Cdc25C (S216) Polyclonal Antibody diluted at 1:1000.
Fig.2. Western Blot analysis of HuvEc cells using Phospho-Cdc25C (S216) Polyclonal Antibody diluted at 1:1000.
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