| Product name | Cdc25A (phospho Ser75) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human Cdc25A around the phosphorylation site of S75 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | CDC25A; M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25A |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 59kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for CDC25A. |
| Gene ID | 993 |
| Alternative | CDC25A; M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25A |
| Others | Phospho-Cdc25A (S75) Polyclonal Antibody detects endogenous levels of Cdc25A protein only when phosphorylated at S75. |
| Accession | P30304 |
| Observed Band(KD) | 59 |
Fig. Western Blot analysis of various cells using Phospho-Cdc25A (S75) Polyclonal Antibody.
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