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CD48 Polyclonal Antibody

CD48 Polyclonal Antibody

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Specification

Product name CD48 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human CD48
Host Rabbit
Reactivity Human,Rat,Mouse,
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:20000;IF 1:50-200
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative CD48; BCM1; BLAST1; CD48 antigen; B-lymphocyte activation marker BLAST-1; BCM1 surface antigen; Leukocyte antigen MEM-102; TCT.1; CD48

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 40kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background CD48 encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The CD48 antigen is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID 962
Alternative CD48; BCM1; BLAST1; CD48 antigen; B-lymphocyte activation marker BLAST-1; BCM1 surface antigen; Leukocyte antigen MEM-102; TCT.1; CD48
Others CD48 Polyclonal Antibody detects endogenous levels of CD48 protein.
Accession P09326
Observed Band(KD) 40

Image & description

Fig.1. Western Blot analysis of K562 cells using CD48 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of K562 cells using CD48 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100.

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