| Product name | CD28 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human CD28 |
| Host | Rabbit |
| Reactivity | Human,Rat,Mouse, |
| Applications | WB,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | CD28; T-cell-specific surface glycoprotein CD28; TP44; CD28 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 25kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | T-cell-specific surface glycoprotein CD28 encoded by CD28 is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Gene ID | 940 |
| Alternative | CD28; T-cell-specific surface glycoprotein CD28; TP44; CD28 |
| Others | CD28 Polyclonal Antibody detects endogenous levels of CD28 protein. |
| Accession | P10747 |
| Observed Band(KD) | 25 |
Fig. Western Blot analysis of K562 cells using CD28 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
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