| Product name | CD19 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human CD19 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,Flow Cyt,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;Flow Cyt 1:50-200;IHC 1:100-500;IF(paraffin section);ELISA 1:5000-20000 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | CD19; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD19 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 61kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. |
| Gene ID | 930 |
| Alternative | CD19; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD19 |
| Others | CD19 Polyclonal Antibody detects endogenous levels of CD19 protein. |
| Accession | P15391 |
| Observed Band(KD) | 61 |
Fig.1. Western Blot analysis of Mouse brain cells using CD19 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Western Blot analysis of Mouse-BRAIN cells using CD19 Polyclonal Antibody diluted at 1:2000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100.
Fig.4. Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100.
Fig.5. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.
Fig.6. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.
Fig.7. Western Blot analysis of Mouse-kidney Mouse-spleen using CD19 Polyclonal Antibody diluted at 1:1500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
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