| Product name | CD180 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human CD180 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | CD180; LY64; RP105; CD180 antigen; Lymphocyte antigen 64; Radioprotective 105 kDa protein; CD180 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 75kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | CD180 is a cell surface molecule consisting of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. The extracellular LRR is associated with a molecule called MD-1 and form the cell surface receptor complex, RP105/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). RP105/MD1, by working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane constituent of Gram-negative bacteria. |
| Gene ID | 4064 |
| Alternative | CD180; LY64; RP105; CD180 antigen; Lymphocyte antigen 64; Radioprotective 105 kDa protein; CD180 |
| Others | CD180 Polyclonal Antibody detects endogenous levels of CD180 protein. |
| Accession | Q99467 |
| Observed Band(KD) | 75 |
Fig.1. Western Blot analysis of Mouse brain cells using CD180 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Western Blot analysis of Mouse-BRAIN cells using CD180 Polyclonal Antibody diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
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