| Product name | CD166 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human CD166 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | IF,WB,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200;WB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications; |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | ALCAM; MEMD; CD166 antigen; Activated leukocyte cell adhesion molecule; CD166 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 65kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | ALCAM encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. CD166 antigen binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. |
| Gene ID | 214 |
| Alternative | ALCAM; MEMD; CD166 antigen; Activated leukocyte cell adhesion molecule; CD166 |
| Others | CD166 Polyclonal Antibody detects endogenous levels of CD166 protein. |
| Accession | Q13740 |
| Observed Band(KD) | 65 |
Fig.1. Western Blot analysis of K562 cells using CD166 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.
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