| Product name | Caveolin-1 (phospho Tyr14) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human Caveolin-1 around the phosphorylation site of Y14 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | CAV1; CAV; Caveolin-1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 20kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The scaffolding protein (caveolin 1) encoded by CAV1 is the main component of the caveolae plasma membranes found in most cell types. Caveolin 1 links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. CAV1 is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in CAV1 have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1. |
| Gene ID | 857 |
| Alternative | CAV1; CAV; Caveolin-1 |
| Others | Phospho-Caveolin-1 (Y14) Polyclonal Antibody detects endogenous levels of Caveolin-1 protein only when phosphorylated at Y14. |
| Accession | Q03135 |
| Observed Band(KD) | 20 |
Fig.1. Western Blot analysis of various cells using Phospho-Caveolin-1 (Y14) Polyclonal Antibody diluted at 1:1000.
Fig.2. Western Blot analysis of HeLa cells using Phospho-Caveolin-1 (Y14) Polyclonal Antibody diluted at 1:1000.
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