| Background |
The caspase 3/7 Active Apoptosis Detection Kit (Green fluorescence) is used for apoptosis detection by measuring the activity of caspase 3/7. Because caspase 3 (CPP32/ Apopain) plays a significant role in the initiation of apoptosis, it has been widely accepted as an indicator of apoptosis. caspase 3 has a specific substrate polypeptide recognition sequence, namely aspartic acid-glutamate-valine-aspartic acid (DEVD).
In this kit, caspase 3/7 Green is conjugated to a high-affinity DNA fluorescent dye using the caspase 3/7 recognition sequence (DEVD), which has cell membrane permeability and can penetrate the cell membrane to enter the cytoplasm. caspase 3/7 Green reagent itself is non-fluorescent and has a charge repulsion effect with DNA. When cells undergo apoptosis, caspase 3/7 Green, as a substrate, is cleaved by activated caspase 3/7 and releases high-affinity DNA dyes. After binding to DNA, it generates strong fluorescence (Ex /Em: 488nm / 525 nm, flow cytometry recommended FITC channel), which is used for the detection of caspase-3/7 activity and apoptosis. This kit can be used to detect the activity and apoptotic status of caspase 3/7 in cells by flow cytometry, fluorescence microplate reader and fluorescence microscope. |
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