| Product name | CARD 10 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human CARD 10 at AA range: 450-530 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | CARD10; CARMA3; Caspase recruitment domain-containing protein 10; CARD-containing MAGUK protein 3; Carma 3 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 120kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]). |
| Gene ID | 29775 |
| Alternative | CARD10; CARMA3; Caspase recruitment domain-containing protein 10; CARD-containing MAGUK protein 3; Carma 3 |
| Others | CARD 10 Polyclonal Antibody detects endogenous levels of CARD 10 protein. |
| Accession | Q9BWT7 |
| Observed Band(KD) | 120 |
Fig. Western Blot analysis of various cells using CARD 10 Polyclonal Antibody.
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